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Journal of Dairy Science Vol. 49 No. 12 1559-1562
© 1966 by American Dairy Science Association ®
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Extended Storage of Microbial Cultures and Culturing Anaerobic Bacteria with Plastic Pouches

E. H. Marth1, J. E. Sherman, L. Hasenzahl and R. V. Hussong

Research and Development Division, National Dairy Products Corporation, Glenview, Illinois

ABSTRACT

Petri-plate, test-tube, and bottle cultures of Pseudomonas fluorescens, Escherichia coli, Lactobacillus casei, Streptococcus lactis, Saccharomyces cerevisiae, Penicillium rogueforti, Leuconostoc citrovorum, Acetobacter aceti, and Clostridium sporogenes were prepared. After growth was sufficient, petri-dish cultures (without the lid) were placed in 4.5-mil K202 plastic bags. Bags were repeatedly flushed with nitrogen, filled with nitrogen to a slight positive pressure, and heat-sealed. Test-tube and bottle cultures were treated in a similar manner, except that caps were removed, placed in the bag loose, and replaced after sealing. Packaged cultures were stored at 7.2 C and examined periodically for viability.

Petri-plate and bottle-slant cultures of all organisms except S. lactis (1.5 months—petriplate; 14 months—bottle-slant) and A. aceti (4 months—both types) remained viable for at least 24 months. Test-tube slant cultures behaved in a fashion similar to that of the bottle slants, except that L. casei lost viability after 20 months of storage. Shake-tube cultures, except for S. lactis (not viable after 1.5 months), behaved as did the test-tube slant cultures.

This technique, when combined with proper incubation of freshly inoculated petri plates, has also been used successfully to culture anaerobic bacteria.

FOOTNOTES

1 Present address: Department of Food Science and Industries, University of Wisconsin, Madison.






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