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Gas-Liquid Chromatographic Analysis of Milk Fat: Rapid Preparation of Butyl Esters¹

Department of Animal Industries, University of Connecticut, Storrs

ABSTRACT

The analysis of milk fatty acids is an arduous task, despite the advantages of modern instrumentation. Various esterification procedures and related techniques have been introduced to help analyze precisely the assortment of fatty acids found in milk fat. Many of these require two different gas-liquid chromatographic (GLC) determinations; one specifically for the short-chain fatty acids. In our laboratory we were most successful with separate GLC determinations of butyl and methyl esters (2). Clement and Bezard (1) also employed butyl esters, but at two different temperatures. Glass et al. (3) have recently published a suitable procedure which employs both ethyl and methyl esters, coupled with temperature programming. Their procedure has the definite advantage of almost instantaneous esterification. We report here on a modification of the latter procedure which possesses the advantage of speed of esteritication and obviates the necessity of two GLC determinations.

The fat to be analyzed is transferred to a suitable vessel.

¹ Scientific contribution no. 208 Agricultural Experiment Station, University of Connecticut, Storrs, supported in part by Public Health Service research grant AM-02605-08 from the Institute of Arthritis and Metabolic Diseases.