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Improved Method for Detection of Microbial Lipolysis

Department of Food Science, University of Illinois, Urbana

ABSTRACT

Many of the proposed plating media for the detection of microbial lipolysis have been based on the dye indicator technique of Turner (10) or the clearing zone technique of Anderson (2). In comprehensive studies of the different media, Jensen and Grettie (5), and more recently Rath (8) were unable to find any of them entirely satisfactory. The major criticisms were: difficulty in interpreting the result, particularly in cases of low lipolytic activity; toxicity of the dyes and some of the fats at the levels employed; and some of the dyes used are oxidation-reduction indicators rather than indicators of fat hydrolysis.

In an effort to minimize some of these difficulties, Rath (8) devised a double-layered agar technique. The basic principle in this method is the same as in the now-classical tallow layer medium of Eijkman (3). One of the layers is designed for the growth of the organisms, whereas the other is prepared solely for the detection of lipolytic activity.