HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Szepesi, B. Articles by Hopwood, M. L. Search for Related Content PubMed Articles by Szepesi, B. Articles by Hopwood, M. L.

Intermediates in the Metabolism of Fructose by Bovine Sperm¹

Endocrine Section, Colorado State University, Fort Collins

ABSTRACT

Metabolites obtained by incubation of washed bovine sperm in citrate-bicarbonate (IVT) buffer were studied using paper chromatographic procedures, U-C¹⁴-fructose, metabolic inhibitors, and standard chemical procedures.

Accumulation of labeled hexose phosphates from fructose was negligible; however, a small amount of fructose-1,6-diphosphate did accumulate in the presence of N-ethyl maleimide and with iodoacetate and adenosine triphosphate. On occasion, a pentose-like substance was found which was not xylose, ribose, arabinose, or ribulose. Its low yield prevented identification. Glucose was formed and positively identified in incubations containing cyanide. It represented about 6% of radioactivity that could be recovered. Results indicate that pathways other than the Embden-Meyerhof may be operative for bovine sperm.

¹ The data are from a thesis presented by the senior author to the Graduate Faculty in partial fulfillment of the requirements for the M.S. degree in Biochemistry.

² Research Trainee in Endocrinology, supported by Grant 5 T1 GM 963 from PHS, National Institute of General Medical Sciences.

³ Present address: Department of Food Science and Technology, University of California, Davis.