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Photometric Determination of Fat in Milk¹

Department of Dairy and Food Science, Cornell University, Ithaca, New York

ABSTRACT

A photometric method for determination of fat in unhomogenized milk was developed. It is based on an indirect measurement of the total amount of light scattered by fat globules. This method has been proved theoretically and experimentally correct.

The wave length of light which gave the maximum value of light scattering was determined by a scanning procedure. From this wave length, _max, the slope of the standard curve was found. If the same standard curve for all milk is preferred, the correct optical path length of an adjustable cuvette may be used.

Fat contents of unhomogenized milk from individual eows, herds, and from mixtures of these milks were measured, representing wide globule size distribution curves. Precision of the photometric method is in between that of the Babcock and Mojonnier methods.

¹ This investigation was supported by U.S. Public Health Service Grant no. AM 0717202.

² Present address: Veksebo, Fredensborg, Denmark.