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Journal of Dairy Science Vol. 49 No. 1 100-102
© 1966 by American Dairy Science Association ®
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Identification of Steroids in Bovine Tissue Using Thin-Layer and Gas Chromatographic Techniques1

W. F. Williams, J. Lynch, M. Barens and B. Fagan

Department of Dairy Science, University of Maryland, College Park

ABSTRACT

The last several years have seen the advent of two powerful new tools for steroid separation and identification; thin layer chromatography (TLC) and gas-liquid chromatography (GLC). Use of these methods has greatly increased the sensitivity of steroid detection and makes possible for the first time an examination of these physiologically important compounds in the target tissues, which have received scant attention as compared to the numerous studies reported for various endocrine organs.

The three tissues examined in this study consisted of nine pooled bovine uteri: five estrual, three luteal, and one pregnant (approximately 75 days); the 18 ovaries from these tracts taken together; and the single placenta. All were obtained at the slaughterhouse as rapidly as possible after the death of the animal, separated, trimmed of extraneous tissue, and placed in chloroform:methanol (2:1, v/v). Upon return to the laboratory, the uteri and ovaries were minced with scissors and Soxhlet-extracted with chloroform :methanol for about 72 hr.

FOOTNOTES

1 Scientific Article no. A1176, Contribution no. 3659 of the Maryland Agricultural Experiment Station, Department of Dairy Science. Supported in part by the Regional Research Project NE-41, A.R.S.—U.S.D.A.; and in part by U.S.P.H.S.—N.I.H. Grant no. GM-11503.






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Copyright © 1966 by the American Dairy Science Association ®.