HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Mikolajcik, E. M. Articles by Gould, I. A. Search for Related Content PubMed PubMed Citation Articles by Mikolajcik, E. M. Articles by Gould, I. A.

Oxytetracycline-Binding Properties of Streptococcus Lactis¹

Department of Dairy Technology The Ohio State University, Columbus

ABSTRACT

Antibiotic-binding differences between an oxytetracycline-resistant and -susceptible strain of S. lactis were studied by use of radioactive tracer techniques. C¹⁴-oxytetracycline was bound tenaciously by both the antibiotic-resistant and antibiotic-susceptible strains. The resistant cells bound a greater quantity of the antibiotic than the susceptible cells, with this binding being particularly marked when oxytetracycline was permitted 3-min contact with the cells. Radioactive oxytetracycline accumulated in the cell fraction, which was sedimented by centrifugation of sonically ruptured cells at 14,000 x g. Oxytetracycline concentration in the cytoplasm of either the resistant or susceptible cells was negligible.

¹ Article 17:63. This investigation was supported by Public Health Services Grant No. EF 00101 from the Division of Environmental Engineering and Food Protection.