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Acid and Alkaline Phosphatase Activity in Bovine Semen as Related to Fertility^{1, 2,}

Department of Animal Industry and Veterinary Science, University of Arkansas, Fayetteville

ABSTRACT

The presence of phosphatase activity in bovine semen has been known for some time and an excellent review of the research has been reported by Mann (3). Bull semen has a very low level of acid phosphatase, but a slightly higher content of alkaline phosphatase. The phosphatase system in the semen of the ram and bull have been studied. Both phosphatases were found to be present, though a difference in the relative proportions of the enzymes was observed in the two species. Bell and Lake (1) recently made a comparison of the phospho-monoesterase activities in the seminal plasmas of the domestic rooster, turkey tom, boar, bull, buck rabbit, and of man. Acid phosphatase activity was highest in man, the rooster, turkey torn, and lowest in the boar and rabbit, with the bull intermediate. Alkaline phosphatase activity was highest in the boar and rabbit, lowest in the rooster, turkey torn, and in man, with the bull again intermediate.

¹ Published with the approval of the Director of the Arkansas Agricultural Experiment Station.

² Supported in part by a grant from the National Association of Animal Breeders.