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Rapid Method for the Determination of Casein in Milkby the Dye Binding Method and for the Detection of Mastitis¹

Department of Animal Sciences, Washington State University, Pullman

ABSTRACT

The casein is removed by buffering a 1:10 dilution of skimmilk at pH 4.6. All operations are performed in a single 50-ml centrifuge tube. After centrifugation, the wet casein precipitate is redispersed and treated with a dye reagent. A standard curve relating absorbance of the remaining free dye to the casein percentage in the original milk is prepared, using reconstituted low-heat, nonfat milk powder of known casein content.

When milks from opposite quarters of the same cow were compared, a highly significant difference in the percentage of casein in the total protein could be attributed to mastitis, as measured by the California Mastitis test. A total of 207 comparisons showed that a CMT-negative reaction corresponded to an average per cent casein of 76. As the CMT reaction increased, there was a 3% decrease in the percentage of casein for each unit increase in the CMT reaction.

¹ Scientific Paper No. 2610. Washington Agricultural Experiment Stations, Project 1631.

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