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Preparation of ß-Lactoglobulin C

Eastern Regional Research Laboratory¹, Philadelphia, Pennsylvania

ABSTRACT

Aschaffenburg and Drewry (1) determined that ß-lactoglobulin of cow's milk occurs in at least two genetically determined variants designated as ß-lactoglobulin A and ß-lactoglobulin B. A third genetically determined variant, ß-C, was recently discovered by Bell (3).

ß-Lactoglobulins A, B, and C have been isolated and crystallized from the milk ofhomozygous cows by the method of Aschaffenburg and Drewry (2). The isolation of ß-C has been modified by Kalan et al. (5, 6) because of its increased solubility.

More than 200 cows of the Jersey breed have been typed for the six possible ß-lactoglobulin phenotypes: A, B, C, AB, AC, and BC. Of the 200, only two cows (one now deceased) havebeen found to produce homozygous ß-C; this illustrates the low frequency of homozygous C. An examination of the milks of 105 Jersey cows of a single herd showed five heterozygote ß-AC's and two heterozygote ß-BC's.

A method for obtaining pure ß-lactoglobulin C from a milk containing ß-lactoglobulins A and C has been developed.

¹ Vermont Agricultural Experiment Station Journal Article No. 517. This study was supported by Vermont Agricultural Experiment Station Project Hatch 289, the Walker Research Fund, and the Windham Research Fund.