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Enumeration of Bovine Spermatozoa by Packed Cell Method¹

Department of Animal Industry and Veterinary Science, University of Arkansas, Fayetteville

ABSTRACT

The enumeration of sperm cells per unit volume of semen is very important in determining the optimum dilution ratio for artificial insemination, in diagnostic laboratories, and as a research tool. There are four methods by means of which concentration of spermatozoa may be estimated with the fourth method being investigated: 1) photoelectric colorimeter method, which is highly repeatable (5, 8) and is used quite extensively and very rapidly; 2) visual determination—opacity rank which gives a rapid estimate, (5) ; 3) hemocytometer method, (5) ; or 4) packed cell method (2, 3, 7). The fourth method employs the packed cells or the hematocrit technique, which is used quite extensively in clinical laboratories as well as in research in determining the per cent red blood cells (RBC) in blood. The application of this technique was investigated as an additional method for enumeration of sperm cells per unit volume.

Semen samples were collected in an artificial vagina from Holstein-Friesian bulls of the Arkansas Agricultural Experiment Station.

¹ Published with the approval of the Director of the Arkansas Agricultural Experiment Station.