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Fate of 4,6-Dinitro-2-Isobutylphenol, 2-Chloro-4,6-Bis(Ethylamino)-S-Triazine, and Pentachloronitrobenzene in the Dairy Cow

Departments of Entomology and Animal Husbandry, Cornell University, Ithaca, New York

ABSTRACT

4,6-dinitro-2-isobutylphenol (DNOSBP) and simazine (2-chloro-4,6-bis-(ethylamino)-s-triazine) may be used for weed control in forage crops. PCNB (pentachloronitrobenzene) controls diseases such as Selerotinea crown rot of red clover. The fate of these compounds was determined in a feeding experiment with catheterized dairy cows.

No residues of these compounds were found in the milk. About 3.5% of intact DNOSBP and 1% of simazine were found in the urine. Pentachloronitrobenzene was largely (45%) eliminated as pentachloroaniline in the urine, as shown by electron affinity gas chromatography.

EXPERIMENTAL PROCEDURE

Four Holstein cows were catheterized and each was fed one of the compounds at the 5-ppm level (based on a daily ration of 50 lb) for three days. The compounds in absolute ethyl alcohol (except simazine which was dissolved in acetone) were mixed with the grain. Morning and evening subsamples of the total mixed milk were taken one day prior to feeding (control sample), daily throughout the feeding period, and for three days thereafter.