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Journal of Dairy Science Vol. 48 No. 3 395-398
© 1965 by American Dairy Science Association ®
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Lipid Classes of Bovine Spermatozoa

Lowell D. Miller and Dennis T. Mayer1

Department of Agricultural Chemistry

Charles P. Merilan2

Department of Dairy Husbandry, University of Missouri, Columbia

ABSTRACT

A modified silicic acid technique was developed for separation of the lipids of bovine spermatozoa into seven distinct classes: saturated hydrocarbons, cholesterol esters, free cholesterol, triglycerides, diglycerides, monoglycerides, and phospholipids. The average lipid content of lyophilized spermatozoa was 11.05%. Monoglycerides and diglycerides were found in exceptionally large quantities, but no unsaturated hydrocarbons of free fatty acids could be detected.

FOOTNOTES

1 Supported in part by Public Health Service Research Grant RG 3180 from the National Institutes of Health.

2 Missouri Agricultural Experiment Station Journal Series No. 2820. Approved by the Director.






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Copyright © 1965 by the American Dairy Science Association ®.