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New Method for Extraction of Milk Phospholipids^{1, 2,}

Department of Dairy Science, Pennsylvania State University, University Park

ABSTRACT

The whole milk sample was compounded with silicic acid, the mixture placed in a glass chromatographic column, and the phospholipid fraction eluted with 20% (v/v) formic acid in ethyl ether. Lipid phosphorus analysis and thin-layer chromatography were used for the comparison of phospholipid recovery from 10-ml aliquots of milk by each of three methods—the new, the Mojonnier, and the modified Mojonnier (1.5% NaCl added to the milk sample before a Mojonnier extraction). Five trials verified that the modified Mojonnier method and new silicic acid column chromatographic (SACC) method recovered 10.2 and 12.2 mean per cent, respectively, more lipid phosphorus than the conventional Mojonnier procedure. When aliquot samples from the phospholipid extracts recovered by the three methods were compared by thin-layer chromatography, minor differences were evident in the lipid patterns, notably in improved recovery of a component corresponding to phosphatidyl serine by the SACC method and in superior recovery of lysophosphatides by the Mojonnier and modified Mojonnier methods.

¹ Authorized for publication as paper No. 2908 in the Journal Series of the Pennsylvania Agricultural Experiment Station, on June 17, 1964.

² Data taken from a thesis presented by A. H. Duthie in partial fulfillment of the requirements for the Ph.D. degree, Pennsylvania State University, March, 1964.

³ Present address: Department of Animal and Dairy Science, University of Vermont, Burlington, Vermont.