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Effect of Lipid Additives on Pre- and Post-Freeze Survival of Bovine Spermatozoa^{1, 2,}

Department of Animal Industries, University of Connecticut, Storrs

ABSTRACT

Bovine spermatozoa were frozen in NJ-2 and EYC extenders with one of seven levels, 0.25, 0.50, 1.0, 2.0, 4.0, 8.0, and 16.0%, of cholesterol, diolein, triolein, milk fat triglycerides, and lecithin. Based upon motility estimates of NJ-2 extended semen, only cholesterol and lecithin provided protection for spermatozoa during the initial cooling phase, and only lecithin protected the cells during freezing and thawing.

In a second study, using 2.0, 4.0, and 8.0% lecithin, with and without 2.0% cholesterol, in citrate, Tris, Minn-Go, phosphate, and NJ-2 buffers, it was found that cholesterol, when averaged across all treatments, reduced motility. It appeared that 2.0% lecithin was superior to 4.0 or 8.0% and that motility was highest in Tris, Minn-Go, and phosphate buffers.

In Experiment 3, 0.5% lecithin, considering all treatments, resulted in greater sperm motility than 1.0 or 2.0%; whereas 5.0% glycerol was superior to either 7.0 or 9.0% prior to freezing, post-thaw motilities were higher with 7.0% glycerol.

In a fourth study, in which semen was extended in 20% EYC or Tris with 1.0% fructose, 0.5% lecithin, and 7.0% glycerol, motility of spermatozoa in EYC was significantly higher (P < 0.01), regardless of freezing method.

¹ Scientific contribution no. 155, Agricultural Experiment Station, University of Connecticut, Storrs. This investigation was supported in part by Public Health Service Research Grant GM 08738 from the National Institutes of Health, Division of General Medical Sciences.

² Portions of the data presented in this paper are from a thesis submitted by the senior author to the Graduate School of the University of Connecticut in partial fulfillment of the requirements for the Master of Science degree.

³ Present address: Department of Anatomy, College of Veterinary Medicine, Iowa State University, Ames, Iowa.