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Structure and Synthesis of Milk Fat. IX. Site of Lipid Synthesis in Freshly Secreted Milk^{1, 2,}

Department of Dairy Science, Pennsylvania Agricultural Experiment Station, University Park

ABSTRACT

In a preceding paper (7) we presented supporting evidence of microsome-like particles in milk (1, 6). This particulate matter exhibited quite uniform lipid composition and occurred both on the fat globules, as globule membrane, and free in the serum of the milk. Present findings indicate that these particles of the serum are the site of the recently reported (5) biosynthesis of glycerides in freshly secreted milk.

EXPERIMENTAL PROCEDURES

Methods of preparing the freshly secreted milk, removing the bulk of its fat, incubating it with tracer fatty acid, separating the various lipid classes, and measuring incorporation of C¹⁴ were performed as in the initial study (5). The milk following incubation with tracer was sedimented in polyethylene centrifuge tubes (35 ml) to separate the particulate matter. These procedures, including the freezing and cutting of the tubes, recovery of lipids from the thawed sections, and thin-layer chromatography of the lipid classes have been previously described (7).

¹ Authorized for publication on June 16, 1965, as Paper no. 3042 in the Journal Series of the Pennsylvania Agricultural Experiment Station.

² Supported in part by the U. S. Public Health Service (HE 03632).