HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Duthie, A. H. Articles by Kendall, R. V. Search for Related Content PubMed Articles by Duthie, A. H. Articles by Kendall, R. V.

New Milk Phospholipid Extraction Method^{1, 2,}

Department of Animal and Dairy Science, University of Vermont, Burlington

ABSTRACT

Milk phospholipids (P-lipids) have been extracted by a new silicic acid column chromatographic (SACC) method (2). This method employs 20% (v/v) formic acid in ethyl ether to elute the P-lipid fraction. Continued investigations on the SACC technique have resulted in several modifications, resulting in more rapid and quantitative recovery.

Raw whole milk samples were obtained from the bulk storage tank in the University Creamery. The extraction methods, manner of handling the samples, lipid phosphorus analysis, and general thin-layer techniques have been previously published (2). One-millimeter chro-matoplates were prepared, using a suspension of 40 g of Silica Gel G (according to Stahl, E. G. Merck, A. G. Darmstadt, Germany) in 80 ml of 0.01 m Na₂CO₃ (3). The P-lipid spots were identified by spraying the plate with the molybdenum blue reagent of Zinzadze, as reported by Dittmer and Lester (1). All organic material was determined by placing the plate (sprayed with molybdenum blue reagent) in a 110 C oven overnight.

¹ This study was supported by Federal Grant, HA-143.

² University of Vermont Agricultural Experiment Station Journal Article no. 157.