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Extraction and Separation of Neutral Milk Lipids by Thin-Layer Chromatography^{1, 2,}

Department of Animal and Dairy Science, University of Vermont, Burlington

ABSTRACT

It is common knowledge that thin-layer chromatography (TLC) is a simple, rapid, and sensitive method for the separation and identification of lipid classes. The samples are invariably applied to the TLC plate as an extract. Recently, Duthie and Patton (1) reported that milk lipids were recovered from whole milk by silicic acid column chromatography. Their research aroused my curiosity and led to the development of a technique whereby milk is spotted directly on a TLC plate, and the neutral lipid group is separated into its respective classes with an appropriate developing solvent.

Raw whole milk samples were obtained from the bulk storage tank in the University Creamery. One-millimeter plates were prepared using a suspension of 40 g Silica Gel G (according to Stahl, E. G. Merck, A. G. Darmstadt, Germany) in 80 ml of 0.01 m Na₂CO₃ (3). Approximately 1 hr before use a plate was activated at 120 C and a developing chamber was saturated with vapors of its solvent.

¹ This study was supported by Federal Grant HA-143.

² University of Vermont Agricultural Experiment Station Journal Article no. 158.