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Contribution of Seminal Plasma, Sperm Numbers, and Gas Phase to Dilution Effects of Bovine Spermatozoa

New Zealand Dairy Production and Marketing Board, A.B. Centre, Newstead, Hamilton

ABSTRACT

Semen stored at 200 million sperm per milliliter at 5 C survived storage significantly better (P < 0.01) than semen stored at 12.5 million. Both additional seminal plasma and dead sperm significantly depressed livability (P < 0.01) in highly diluted samples. However, this difference between dilution rate of storage was eliminated when diluents were saturated with nitrogen, and a decline in storage life, similar to that exhibited by highly diluted sperm, could be simulated in sperm stored at 300 million per milliliter in a diluent gassed with oxygen. It is concluded that the dilution effects observed are due to availability of O₂ to sperm, and that the protective action of nitrogen saturation lies in the reduction of O₂ tension in the diluting media. Field trials showed an advantage for storage at 200, but rediluted to 12.5 million sperm per milliliter immediately before use, compared to storage at 12.5 million sperm per milliliter for semen used on the day after collection (difference 3.1% P < 0.025) in an unsaturated diluent, but an advantage of 11.7% (P < 0.01) in favor of storage at 12.5 million sperm per milliliter with semen used seven days after collection in a nitrogen-saturated diluent.