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Isolation of Ribonucleic Acid from Cow Mammary Gland¹

Laboratory of Biochemistry, Department of Dairy Science, University of Illinois, Urbana

ABSTRACT

There is increasing evidence that various forms of ribonucleic acid (RNA) perform essential roles in the transfer of genetic information and protein synthesis (10). Though there have been some studies on the isolation of and the base composition of RNA from rat mammary gland (2, 9), little attention has been paid to the isolation of RNA from cow mammary gland. Some procedures employed successfully to isolate rat liver RNA were examined for the isolation of RNA from cow mammary gland. Results are presented in this paper.

EXPERIMENTAL PROCEDURE

Pieces of cow mammary gland were removed from the udder immediately after slaughter and frozen in dry ice. In most cases, the extraction of RNA was done immediately after sampling. In other cases, the mammary gland was stored at –20 C; 20–200-g pieces of the mammary gland were minced by a mechanical chopper or scissors into small pieces before extraction. The following three procedures for the isolation of RNA were examined:

¹ This investigation was supported in part by a Grant from the National Science Foundation.

² On sabbatical leave (1962–63) from the National Institute of Animal Industry, Chiba-Shi, Japan.