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Evidence for Loss of Lipid from Bovine Spermatozoa due to Freezing¹

Department of Animal Industries, University of Connecticut, Storrs

ABSTRACT

The 1.26 mg/ml of lipid extracted from seminal plasma separated from spermatozoa prior to freezing (prefreeze) was lower (P < 0.01) than the 1.92 mg/ml obtained from seminal plasma separated following freezing of the whole raw semen. There was also a significant difference (P < 0.05) between the 2.34 and 1.63 mg/ml extracted from the pre- and post-freeze spermatozoa. Freezing of whole raw semen prior to separation apparently results in an alteration in the quantity of lipid found in the cells and seminal plasma. Possible alteration of lipid classes due to freezing was also observed.

¹ Scientific contribution No. 90, Agricultural Experiment Station, University of Connecticut, Storrs. This investigation was supported in part by Public Health Service Research Grant GM 08738 from the National Institutes of Health, Division of General Medical Sciences.