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Fractionation of the Bovine Ejaculate¹

Animal Reproduction Laboratory

J. F. Masken and M. L. Hopwood

Endocrine Section, Colorado State University, Fort Collins

ABSTRACT

The contribution of the accessory sex glands to the bovine ejaculate has been investigated (2, 4, 5) to determine the relationship of endocrine patterns to accessory sex gland function. The role of the accessory sex glands in reproduction is not well-defined. Alterations in the function of these glands may or may not be related to fertility.

Separating the ejaculate into well-defined components would enhance our knowledge of accessory gland function. The bull has presented a problem in this respect, since the bovine ejaculatory process is said to be instantaneous (6). Lutwak-Mann and Rowson (6) were able to collect two well-defined fractions of bull semen using electric stimulation, but felt that it was not feasible to collect separate fractions by means of the artificial vagina.

This study was conducted to determine the possibility of fractionating bovine semen during collections with the artificial vagina and to compare these to fractions collected by electro-ejaculation.

¹ Published with the approval of the Director of the Colorado Agricultural Experiment Station as Scientific Series Paper No. 948. This investigation was supported by a PHS Research Grant, GM 07120.