HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Steinbach, J. Articles by Foote, R. H. Search for Related Content PubMed Articles by Steinbach, J. Articles by Foote, R. H.

Effect of Catalase and Anaerobic Conditions upon the Post-thawing Survival of Bovine Spermatozoa Frozen in Citrate- and Tris-buffered Yolk Extenders¹

Department of Animal Husbandry, Cornell University, Ithaca, New York

ABSTRACT

Semen samples from six different bulls were extended in citrate-fructose-yolk-glycerol (CFYG) and Tris-fructose-yolk-glycerol (TFYG) extenders with and without catalase, sealed under a high partial pressure of purified nitrogen or argon gas, and equilibrated with glycerol for 6 or 18 hr, and then frozen at rates of 0.8, 3.0, and about 8.0 C per min in the critical region from 5 C to –15 C. The mean percentages of motile cells in TFYG and CFYG were, respectively, 36 and 31 immediately after thawing and 31 and 22 after a post-thawing storage period of 24 hr at 5 C. Thus, the percentage of motile cells in TFYG 24 hr after thawing was equal to that found in freshly thawed CFYG. Neither catalase nor the inert gases exerted a significant influence upon post-thaw recovery of motile spermatozoa, although in the absence of catalase argon gassing showed a positive effect during the period of liquid storage. The slight differences between the periods of glycerol equilibration and among freezing rates were not significant.

¹ Supported in part by the New York Artificial Breeders' Cooperative, Inc.

² Present address: Institut fur Tierzücht und Haustiergenetik der Universitat Göttingen, Germany.