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Metabolism of Urea Nitrogen by Mixed Cultures of Rumen Bacteria Grown on Cellulose^{1, 2, 3, 4,}

Department of Animal Science, Ohio Agricultural Experiment Station, Wooster

ABSTRACT

The incorporation of urea nitrogen into protein and other nitrogenous compounds by mixed cultures of rumen bacteria grown on cellulose was studied in vitro, using trichloroacetic acid (TCA) and tungstic acid (TA) as protein precipitants. Tungstic acid precipitated more protein from the in vitro rumen fermentation than did trichloroacetic acid. TCA was shown to precipitate mainly cellular debris, whereas tungstic acid precipitated the soluble cellular and extracellular proteins as well. Total nitrogen in the in vitro fermentation mixture was accounted for in the following nitrogenous fractions: protein, peptide, amide, ammonia, and a trace of residual urea. Nitrogenous fractions precipitated by tungstic acid gave a better estimate of bacterial growth. Synthesis of this fraction followed more closely the build-up of total cell nitrogen and was more highly correlated with bacterial activity than the nitrogenous fraction precipitated by trichloroacetic acid. The growth-limiting effect of low valeric acid fermentations was reflected in the amounts of cytoplasmic proteins synthesized, but the build-up of cellular debris was not restricted.

¹ Approved for publication as Journal Article No. 97-63 by the Associate Director of the Ohio Agricultural Experiment Station, Wooster, Ohio.

² Data taken from portions of a dissertation submitted by the senior author to the Graduate School of The Ohio State University in partial fulfillment of the requirements of the degree of Doctor of Philosophy.

³ Preliminary reports of this work were presented at the annual meetings of the American Society of Animal Science, 1961 and 1962.

⁴ This research was supported in part by a Grant-in-Aid of research from the Nitrogen Division, Allied Chemical Corporation, New York.

⁵ Present address: Animal Research Institute, Research Branch, Canada Department of Agriculture, Ottawa, Ontario.

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