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Journal of Dairy Science Vol. 47 No. 7 758-760
© 1964 by American Dairy Science Association ®
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Anaerobiosis Produced by Glucose Oxidase to Control Growth of Test Bacteria in Antibiotic Assays1

R. A. Ledford2 and F. V. Kosikowski

Department of Dairy and Food Science, Cornell University, Ithaca, New York

ABSTRACT

Methylene blue studies revealed traces of oxygen in solidified agar which was subjected to the method outlined by Kosikowski; however, Clostridium sporogenes grew slowly under these conditions. This residual oxygen was rapidly removed by reactions catalyzed by glucose oxidase and catalase. Substituting sodium thioglycollate for glucose oxidase was without effect. Inclusion of catalase and proper buffering capacity prevented any antibacterial action of glucose oxidase. Clostridium sporogenes grew rapidly under this technique, which provided anaerobiosis for individual petri dishes.

FOOTNOTES

1 This research was supported in part through Public Health Service Grant EF-00003-06, Division of Environmental Engineering and Food Protection.

2 Present address: Director, State Food Laboratory, New York State Department of Agriculture and Markets, Albany, New York.






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Copyright © 1964 by the American Dairy Science Association ®.