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Genetic Polymorphism in Caseins of Cow's Milk. IV. Isolation and Properties of ß-Caseins A, B, and C

Eastern Regional Research Laboratory,¹ Philadelphia, Pennsylvania

ABSTRACT

A convenient method is presented for obtaining the genetic variants of ß-casein of high purity utilizing DEAE-cellulose chromatography in the presence of 3.3 M urea as a dissociating agent. Furthermore, the separation of heterozygous mixtures of the ß-casein variants may be accomplished with column chromatography by simple manipulation of salt elution gradients. ß-Caseins A, B, and C contained 15.18, 15.33, and 15.45% nitrogen, respectively, and 0.59, 0.57, and 0.50% phosphorus. The genetic forms of ß-caseins may be conveniently identified by their relative electrophoretic mobilities on starch-gel and polyacrylamide-gel.

¹ Eastern Utilization Research and Development Division, Agricultural Research Service, U. S. Department of Agriculture.