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s- and ß-Casein VariantsEastern Regional Research Laboratory1, Philadelphia, Pennsylvania
ABSTRACT
In 1962, we reported the discovery of genetic variants of
s-casein of cow's milk (7). Three electrophoretic forms of
s-casein can occur singly (A, B, or C) or in pairs (AB, AC, or BC) in the milk of individual cows, an observation which parallels the studies of Aschaffenburg (1) on ß-caseins. The genetics of
s-casein polymorphism is described by Kiddy et al. (5).
This report describes some of the characteristics of purified
s- and ß-caseins. Additional properties and a more detailed report will appear in a subsequent publication. Each of the genetic variants of
s- and ß-caseins was prepared from the milks typed homozygous for the particular variant. All but ß-casein C were available, and whole casein of this type was a gift of Dr. R. Aschaffenburg, Reading, England.
Briefly, the preparation of
s-casein involved the isolation of whole
-casein by the method of Hipp et al. (3), followed by the separation of crude
s-casein with calcium ions at 0–4 C.
1 Eastern Utilization Research and Development Division, Agricultural Research Service, U. S. Department of Agriculture.
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