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Department of Dairy and Food Industry Iowa State University, Ames
ABSTRACT
Concern has been expressed that all microorganisms recovered from selective media used to enumerate coliforms might not be conventional Escherichia or Aerobacter, but could include considerable numbers of Erwinia. Microorganisms commonly isolated from Violet red bile (VRB) agar have been identified as Escherichia, Aerobacter, and intermediate types, all of which are considered common inhabitants of manure, soil, grain, and vegetable matter (1). Erwinia, while closely related to conventional coliforms, are plant pathogens usually isolated from vegetable matter (1) and bear no enteric connotation.
Several workers have noted similarity between Erwinia and the more common coliform organisms (3–5). The possibility of Erwinia in milk can be seen when the proximity of forage to the milking operation is considered. Furthermore, the addition of fresh and dehydrated vegetables to Cottage cheese, dairy dips, and spreads has become prevalent. Erwinia, appearing as typical coliform colonies in VRB agar, might result in misinterpretation of the significance of coliform counts obtained from these products.
1 Journal Paper No. J-4915 of the Iowa Agricultural and Home Economics Experiment Station, Ames, Iowa. Project No. 1488.
2 Data taken from a thesis submitted by the senior author to the Graduate College of The Iowa State University in partial fulfillment of the requirements of the degree Master of Science.
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