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Journal of Dairy Science Vol. 47 No. 12 1403-1406
© 1964 by American Dairy Science Association ®
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Bovine Semen Frozen in Liquid Nitrogen Vapor1

J. D. Roussel, H. C. Kellgren and T. E. Patrick

Department of Dairy Science, Louisiana State University, Baton Rouge

ABSTRACT

Laboratory studies employing the split-ejaculate technique from eight mature bulls were used to evaluate the effects of per cent glycerol levels of 6, 7, 9, and 11 and equilibration time of 6, 8, 12, and 18 hr frozen in liquid nitrogen vapor (LNV). Highly significant differences (P < 0.01) between equilibration time (hours) and per cent glycerol levels were found. A further breakdown of the analysis showed that 7% glycerol at 12 hr equilibration was superior (P < 0.05) to the remaining treatments.

In the fertility study, split ejaculates from seven mature bulls (two Jersey, three Holstein, one Guernsey, and one Angus) were used to study the effects of freezing semen in LNV and dry ice and alcohol. The mean prefreezing and post-thawing motility values were essentially the same for both treatments. Also, differences between treatments after 3 wk storage were not significantly different (P > 0.05).

Fertility based on 60- to 90-day nonreturns for 824 first services was 76.1% for the semen frozen in LNV, as compared to 75.5% for 853 first services for the control. These differences were not significantly different (P > 0.05). A significant (P < 0.05) simple correlation was found between fertility and motility after 5 hr incubation at 38 C.

FOOTNOTES

1 This investigation was financially supported by the Louisiana Artificial Breeding Cooperative, Incorporated, and Louisiana Agricultural Experiment Station, Baton Rouge.

2 Present address: Department of Animal Industry and Veterinary Science, University of Arkansas, Fayetteville.






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