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Journal of Dairy Science Vol. 47 No. 11 1220-1223
© 1964 by American Dairy Science Association ®
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Pectic Enzymes of Rumen Fluid1

W. W. G. Smart, Jr., T. A. Bell, E. D. Mochrie and Nancy W. Stanley

Departments of Animal Science and Food Science, North Carolina State of the University of North Carolina at Raleigh

ABSTRACT

A glycosidic pectic enzyme was found in a number of rumen fluid samples and was characterized as {gamma}-pectinglycosidase. It was exocellular and adaptive to the addition of pectic substances, with a high activity in the pH range of 7.0 to 9.5 (optimum 8.5). The rumen fluid was free of pectinesterase as determined at pH values of 4.5, 6.0, and 7.5. When a basal ration of four fistulated steers was supplemented with citrus pulp there was a tenfold increase in activity of {gamma}-PG activity attributed to citrus pulp. There was no difference between the levels of citrus pulp used.


FOOTNOTES

1 This study was conducted under a cooperative project between North Carolina Agricultural Experiment Station and U. S. Food Fermentation Laboratory of Southern Utilization Research and Development Division, ARS, USDA. Published with the approval of the Director of Research, North Carolina Agricultural Experiment Station as Paper No. 1798 in the Journal Series.







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Copyright © 1964 by the American Dairy Science Association ®.