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Collection of Blood from the Cavernous Sinus in the Cow

Cornell University, Ithaca, New York

ABSTRACT

Fluhman (7) first demonstrated pituitary gonadotropins in human blood when he injected 3.0–5.0 ml serum from ovariectomized women into infantile mice producing follicles and corpora lutea. Several authors (1, 3) have described methods of fractionation of plasma proteins with the isolation and detection of the gonadotropic fraction. Apostolakis (2) has described a method of concentration of plasma rendering it less toxic to bioassay animals. The method involved precipitation with acetone and dialyzing against normal saline.

Ganong and Hume (8), using a surgical approach, collected cavernous sinus blood in the dog and demonstrated the presence of ACTH. McFarland, Clegg, and Ganong (10) and Ellington, Contopoulos, and Clegg (6) were able to detect corticotropin gonadotropic, and growth hormone activities in cavernous sinus plasma collected from gonadectomized, unanesthetized sheep. The blood was collected by passing a needle through the foramen ovale.

In sheep, Daniel and Prichard (5) have shown that much of the pituitary venous drainage is into the cavernous sinus, and a similar situation has been observed in the cow [Cummings (4)].