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Some Immunological Relationships of -Lactalbumin and ß-Lactoglobulin in Milks of Various Species¹

Laboratory of Biochemistry, Department of Dairy Science, University of Illinois, Urbana

ABSTRACT

Separate rabbit antisera prepared against cow -lactalbumin and ß-lactoglobulin were found in Oudin diffusion tests and on Ouchterlony plates to react with the milk of some, but not all, of a variety of species. A high degree of cross reaction was found with the milks of other ruminants (goat, sheep, and water buffalo), but not with the milks of the nonruminants tested (camel, horse, rat, mouse, guinea pig, pig, dog, and rabbit). The antiserum to cow ß-lactoglobulin reacted with sheep milk about three times as strongly as with cow, goat, and buffalo milk, and the antiserum to cow -lactalbumin reacted about twice as strongly with the goat and buffalo milk as with cow or sheep milk. Comparative analyses using crystalline ß-lactoglobulins isolated from cow, sheep, and goat milks indicated that the antiserum to cow ß-lactoglobulin reacted more strongly with sheep and goat ß-lactoglobulin than with cow ß-lactoglobulin itself. When the analyses are corrected on this basis, it is possible to analyze sheep and goat (and probably buffalo) milk for their ß-lactoglobulin contents by using the antiserum to cow ß-lactoglobulin. These results appear to agree with the contents of ß-lactoglobulin indicated in these milks by other procedures.

¹ This investigation was supported in part by a grant from the National Science Foundation.

² On sabbatical leave (1962–3) at the University of Illinois from the National Institute of Animal Industry, Chiba-Shi, Japan.

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