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Joint Collaborative Study of the Direct Microscopic Clump Count Method in Dry Milks and its Statistical Considerations

American Dry Milk Institute, Chicago, Illinois

E. Small

Dairy Division, A.M.S., U. S. Department of Agriculture, Washington, D. C.

ABSTRACT

A study was condueted with 23 laboratories for determining and analyzing the variability of the Direct Microscopic Clump Count, as applied to dry milks.

Results obtained were analyzed statistically. Between laboratories results indicated wide variation. Within laboratories results were generally fairly reproducible for all levels of count when using the Levowitz-Weber single solution stain. For the aniline oil-methylene blue stain the average coefficient of variability within laboratories was higher.

The Levowitz-Weber single solution stain yielded higher counts than the aniline oil-methylene blue stain. No substantial differences in variability were found for the laboratories arranged according to their experience with a given stain, or when the routine laboratories were compared with the nonroutine laboratories. There were no outstanding differences in the reproducibility of results, either within or between laboratories when the DMCC procedure was applied to spray process nonfat dry milk, roller process nonfat dry milk, and spray process dry whole milk.

¹ Present address: The Hunt Foods and Industries, Inc., Fullerton, California.