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Electron Microscopic Observations of Alkaline Earth Metal-Caseinate Particles

Laboratory of Animal Products Technology, College of Agriculture, Tohoku University, Sendai, Japan

ABSTRACT

It has been well established that casein exists in milk as roughly spherical particles of varying sizes (1, 3, 7, 8) and containing calcium and magnesium (4). The effects of the metals on the particle formation and size distribution in complexing with casein have stimulated further interest in the behavior of the caseinate system. This paper presents evidence that when alkaline earth metal chlorides are added to casein solutions, the size of the particles depends upon the particular alkaline earth metal added.

Suspensions of the alkaline earth metal-caseinates were prepared by adding the following amounts of 0.1 M alkaline earth metal chloride solutions to 100 ml of 0.01% casein solutions: 0.5 ml of barium chloride, 0.25 ml of beryllium chloride, 1 ml of calcium chloride, 1 ml of magnesium chloride, 0.25 ml of strontium chloride. They were allowed to stand for 2 hr. The casein was obtained from cow's milk according to the directions of Warner (10) and dissolved in 0.01 N sodium hydroxide solution, then adjusted to pH 7.0 with dilute hydrochloric acid.