HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Nielsen, E. K. Articles by Hussong, R. V. Search for Related Content PubMed Articles by Nielsen, E. K. Articles by Hussong, R. V.

Determination of Heat-Induced Lactose-Protein Combination in Milk with Lactose-1-C¹⁴

Fundamental Laboratory, Research and Development Division, National Dairy Products Corporation, Glenview, Illinois

ABSTRACT

A tracer study was made to determine how much of the lactose in cow's milk combined with the protein fraction on heating. This was done by adding an aqueous solution of lactose-1-C¹⁴ to fresh skimmilk, allowing it to equilibrate before heating, isolating protein, by acid precipitation or exhaustive dialysis, and counting the C¹⁴ in the isolate. Protein isolated by either method contained C¹⁴ tracer, indicating a protein-lactose interaction to form a stable complex. The protein fraction of raw skimmilk was found to contain 0.03% of the added tracer. Heating the dosed skimmilk at 145 and 208 F for 30 min resulted in the presence of 0.06 and 0.7% of added tracer in the protein fraction. These values, then, represented the percentages of lactose, either in the form of intact lactose or radioactive fragments thereof, combined with the protein as a result of the heat treatments accorded.