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Turbidimetric Method for Estimating Sum of ß- and -Caseins in Whole Casein¹

Division of Applied Biology, National Research Council, Ottawa, Canada

ABSTRACT

The amount of ß + -casein present in whole casein was estimated in a buffered solution of acid casein in the presence of calcium, by means of the difference in optical densities (400 mµ) at 0 and 35 C; the concentration of _s-casein was obtained by difference. By subtracting the -casein content (estimated by sialic acid analysis), the amount of ß-casein present in the ß + -casein mixture was obtained.

Analysis of various test solutions containing known amounts of _s-, ß-, and -caseins gave results which agreed within ±2.3%. The method is simple, convenient, and suitable for routine determinations. Analysis of caseins obtained from individual lots of skimmilk indicated that ß-casein accounted for 19–36%, -casein for 10–23%, and _s-casein for 49–60% of the total casein.

¹ Issued as N.R.C. No. 7483.