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Method of Biopsy of the Mammary Gland of Cows and Goats¹

Department of Dairy Husbandry, University of Minnesota, St. Paul

ABSTRACT

Many attempts have been made to obtain tissue samples from the bovine mammary gland for histological studies. Hedstrom (2) obtained mammary tissue with a trocar composed of a metal tube holding a sliding knife which was inserted through an incision in the mammary gland. Turner and Reineke (5) obtained mammary gland biopsies through a 2-in. incision. The blood vessels were ligated with cat-gut and the tissue desired was tied off with suture and removed with a scalpel. Following biopsy a large number of the biopsied sites were infected. The authors attributed this infection to leakage of milk into the biopsied site. Andberg et al. (1) used a sharp brass cork borer inserted through an incision made in the skin to obtain udder tissue. Porter and Petersen (4) obtained mammary tissue biopsies with a needle which terminated in a single cutting edge 90° to the horizontal axis of the needle. This cutting needle was inserted through an incision in the mammary gland.

¹ Paper No. 5049, Scientific Journal Series, Minnesota Agricultural Experiment Station.