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Effect of Phosphate and of Related Co-factors on the Metabolism of Bovine Spermatozoa

Department of Dairy Science, University of Illinois, Urbana

ABSTRACT

Experiments were conducted to reassess the effect of diluents containing fructose and seminal plasma on the Pasteur effect in bovine spermatozoan metabolism. When diluted semen was studied, the Pasteur effect was more marked in saline than in phosphate diluent. With twice-washed spermatozoa a substantial Pasteur effect was demonstrated in phosphate but not in saline, for only a minimum of lactic acid accumulated in saline even under anaerobiotic conditions. The addition of low levels of inorganic phosphate (levels which did not inhibit O₂ uptake), adenosine diphosphate and combinations of these, as well as the addition of 0.002 M oxidized or reduced diphosphopyridine nucleotide, did not stimulate the low lactic acid accumulation by washed sperm cells diluted with saline. High levels of phosphate in the diluent were stimulatory to fructolysis and inhibited the oxidation of pyruvate and lactate.