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Journal of Dairy Science Vol. 46 No. 5 444-446
© 1963 by American Dairy Science Association ®
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Analysis of Biological Fluids for Carbon Tetrachloride after its Administration into the Bovine Rumen1

R. Bassette, S. Özeris, E. E. Bartley and I. S. Yadava

Department of Dairy Science, Kansas State University, Manhattan

ABSTRACT

To develop techniques to study transmission of volatile materials to milk, a cow was given 4 g CCl4 per os. Carbon tetrachloride was used, since it is not metabolized and can be detected readily by gas chromatography. The CCl4 was observed in milk, blood, and urine 15 min after administration. Its concentration in these fluids increased gradually during the first hour, reached a peak on the first and second day, and had practically disappeared by the third day. Changes in normal concentration of acetone in milk, blood, and urine were observed also. The techniques employed suggest a method for studying transmission of volatile materials to milk.

FOOTNOTES

1 Contribution Number 312, Kansas Agricultural Experiment Station.






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Copyright © 1963 by the American Dairy Science Association ®.