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Livability of Bovine Spermatozoa at 5, –25, and –85 C in Tris-Buffered and Citrate-Buffered Yolk-Glycerol Extenders

Department of Animal Husbandry, Cornell University, Ithaca, New York

ABSTRACT

Survival of bovine spermatozoa in 0.2 and 0.25 M Tris-buffered yolk-glycerol (TYG) extenders was generally superior to that in a standard citrate-yolk-glycerol (CYG) extender at 5, –25, and –85 C. The 0.1 and 0.15 MTris-buffered extenders were inferior, P < .01, and the bending of the sperm's tails in these two extenders suggested that these levels of Tris were hypotonic. Twenty ejaculates of semen, averaging 62% motile sperm initially, averaged 50 and 48% af ter eight days' storage at 5 C in 0.2 M and 0.25 M TYG, respectively. These same extenders averaged 31% or less motile sperm after one day of storage at –25 C, indicating that this temperature was unsatisfactory for sperm storage with the extenders used. At –85 C, nearly 90% of the sperm motile before freezing were still motile immediately after freezing in 0.2 M and 0.25 M TYG, but this percentage had dropped to 66% sixty days after freezing. This latter survival rate was higher, P < .05, than that observed in the standard CYG. The accidental exposure of stored samples from eight ejaculates for a brief period to a temperature warmer than –85 C suggested that such temperature changes were less critical in their effects on sperm survival in the Tris-buffered than in the citrate-buffered extenders.

¹ Present address: Cornell University Medical College, New York, New York.

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