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Response of Some Blood Constituents to Infusions of Disodium Ethylenediamine Tetraacetate in Intact Cattle^{1, 2,}

Department of Dairy Science, University of Arizona, Tucson

ABSTRACT

Lactating and dry cows were intravenously infused with a 10% aqueous solution of disodium ethylenediamine tetraacetate. Infusion was at a constant rate until tetany developed. Blood samples were drawn before infusion and at specified times during and post-infusion. The serum was analyzed for calcium and inorganic phosphorus. Total leucocytes were counted. Smears were made from each blood sample and stained for differential counts. The means are from 19 observations (13 for the leucocytes). The serum calcium mean preinfusion level was 10.79 mg per 100 ml, decreasing to a mean of 4.9 mg per 100 ml at tetany. Tetany occurred at a mean of 163 min from the beginning of infusion. At 24-hour post-infusion the mean was 10.11 mg per 100 ml. The mean preinfusion level of phosphorus was 6.10 mg per 100 ml and the mean level at tetany was 4.28 mg per 100 ml. The low mean of phosphorus, 3.89 mg per 100 ml, occurred 90 min post-tetany. The preinfusion levels of phosphorus were practically attained by 12-hr post-infusion. A slight decrease followed by a steady increase to a peak at 8-hr post-infusion occurred in the total WBC count. There was a gradual decrease in number from the peak to the 24-hr sample. A sharp diminution in the per cent of lymphocytes occurring simultaneously with a marked increase in the per cent of neutrophils caused a crossing over in the percentages of these types of cells by the 3-hr post-infusion sampling. Normal ratios had not been attained by 24-hr post-infusion.

¹ This work was supported in part by Grant No. A-4043 from the United States Public Health Service.

² Arizona Agricultural Experiment Station, Technical Paper No. 745.