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Changes with Age in Glutamic Oxalacetic Transaminase Activity of Sonically Oscillated Rumen Juice Compared to Total Steam Volatile Fatty Acids in Calves Fed Different Roughages¹

Department of Dairy Science, Ohio Agricultural Experiment Station, Wooster

ABSTRACT

Glutamic Oxalacetio Transaminase (GOT) measurement has proven to be a useful clinical blood test for detecting cellular breakdown, especially myocardial damage and liver necrosis. In this test, the GOT released from the damaged cells into the blood plasma is determined.

In a preliminary experiment, the concentration of GOT activity was found to he highest in the sonieally oscillated protozoal fraction, next highest in the oscillated bacterial fraction, and lowest in the cell-free fraction of fresh rumen juice taken from a fistulated steer fed a ration of alfalfa hay. This observation suggested the possible usefulness of GOT measurement in sonically oscillated rumen juice as a means for following the microbial development in the rumen related to age and ration in young calves.

In this experiment, one of three roughages, alfalfa, soybran flakes, or beet pulp was used as the basic roughage in the pelleted rations fed the three groups of calves (2). The amounts of the different roughages on the air-dry basis were, respectively, 66.7, 56.7, and 56.7% of the total ration.

¹ Approved for Publication as Journal Article No. 91-62 by the Associate Director of the Ohio Agricultural Experiment Station, Wooster.