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Evidence for Presence oF Hydrocortisone in Cow Urine

Kyushu Agricultural Experiment Station, Ministry of Agriculture and Forestry, Japan

Shoko Eto

Faculty of Pharmacology, University of Kumamoto

ABSTRACT

A few authors have reported 17-hydroxycorticosteroids in urine of the bovine. Unger et al. (10) reported a large quantity of nonsteroid Porter-Silber reactive chromogens (8) with a small amount of corticosteroids in the ethyl acetate extract of urine from six calves. The nonsteroid substances were supposed to be the ionone derivatives which were reported by Holtz (6) and Mixner et al. (7) in their studies of 17-ketosteroids. Holm and Firch (5) studied the 17-hydroxycorticosteroids in cow urine, and reported that they could find the corticosteroids in some breeds of the cow. On the other hand, Wynne (11) could not find any endogenous corticosteroids in the urine from ewes.

None of those authors reported 17-hydroxycorticosteroids in cow urine. Only Unger et al. (10) suggested the presence of tetrahydrocortisol in cow urine by paper chromatography. For these reasons, the identification of cortisone and hydrocortisone in bovine urine was studied.

Urine was obtained from a healthy Holstein cow that weighed 575 kg, produced 15 kg of milk daily, and was nonpregnant.