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Elimination of 2,4-D in the Urine of Steers Fed 4-(2,4-DB) or 2,4-D

Departments of Entomology and Animal Husbandry, Cornell University, Ithaca, New York

ABSTRACT

In earlier studies, the herbicides 4-(2,4-dichlorophenoxybutyric) acid [4-(2,4-DB)] and 2,4-dichlorophenoxyacetic acid (2,4-D), were fed to dairy cows and were shown to be absent in the feces and milk (1, 2). In the work reported, 4-(2,4-DB) was shown to be eliminated in the urine of a steer largely as 2,4-D. A second steer, when fed 2,4-D, eliminated the intact compound in its urine.

EXPERIMENTAL PROCEDURE

A Holstein steer was fed 5 ppm (based on a daily ration of 50 lb) of 4-(2,4-DB) on October 8, 1963. The pure herbicide in ethyl alcohol was mixed with the grain. A second steer was similarly fed 5 ppm of 2,4-D. The steers were kept in special stalls allowing for separate urine or feces collection, or both. Total urine samples were weighed and collected the day before feeding (control sample) and daily for seven days after feeding the herbicides.

The method of analysis consisted of blending 25 g of the well-mixed, filtered, daily total urine sample with 70 ml of acetone and 1 ml of 85% phosphoric acid.