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Journal of Dairy Science Vol. 46 No. 11 1278-1282
© 1963 by American Dairy Science Association ®
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Influence of Nitrogen and Argon Gases on Post-Thawing Motility, Laboratory Stress Tests, and Fertility of Frozen Bovine Spermatozoa1

J. D. Roussel2, T. E. Patrick, H. C. Kellgren and J. O. Shelwick

Department of Dairy Science, Louisiana State University, Baton Rouge

ABSTRACT

Flushing ampules with N2 gas was found to be superior to flushing with A or no flushing on post-thawing motility and livability at 38 C in the first experiment.

In the second experiment, the effects of N2 flush were tested on prefreezing motility, post-thawing motility, livability at 38 C, and fertility. Prefreezing means were essentially the same for the control and N2 flushing. However, a significant difference (P < 0.01) between treatments was obtained for the post-thawing motility, with mean values of 45.5 and 41.7% for the N2 flush and control, respectively. Semen treated with N2 survived considerably better (P < 0.01) than the control for the livability at 38 C. A slight but significant (P < 0.05) increase in lactic acid production was observed at post-thawing and after the livability at 38 C for semen treated with N2.

Fertility based on 60- to 90-day nonreturns for 1,905 first services was 74.50% for N2 flushed semen as compared to 71.87% for 1,930 first services for the control. These differences did approach significance (P < 0.05). A highly significant (P < 0.01) simple correlation coefficient of +0.79 was found between fertility and motility after incubation at 38 C.

FOOTNOTES

1 This investigation was financially supported by the Louisiana Artificial Breeding Cooperative Incorporated.

2 Present address: Department of Animal Industry and Veterinary Science, University of Arkansas, Fayetteville.






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