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Purification and Some of the Properties of _s-Casein and -Casein

Eastern Regional Research Laboratory,¹ Philadelphia, Pennsylvania

ABSTRACT

_s-Casein and -casein have been freed of major contaminants, judged by starch-gel-urea electrophoresis. The _s-casein was prepared by a urea-sodium chloride procedure with a final precipitation of impurities by ethanol. -Casein was prepared by extraction of whole casein with urea-sulfuric acid supplemented with ethanol precipitation. Electrophoretic mobility, pH of minimal solubility salt-free (isoionic point), and with 0.1 M NaCl (isoelectric point), ultraviolet absorption, and interaction with calcium ions are reported.

¹ Eastern Utilization Research and Development Division, Agricultural Research Service, U. S. Department of Agriculture.

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