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Separation of Organic Acids from Ruminant Blood by the Wiseman-Irvin Method¹

Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

A modification of the Wiseman-Irvin chromatographic method (9) has been used extensively in this laboratory for separating organic acids from ruminant blood and forestomachs' ingesta. The techniques employed are patterned after those first developed for ruminant blood and rumen fluid analysis by McCarthy (6). The objectives of this paper are to report conditions found favorable for the separation of the short-chain organic acids of ruminant blood by the Wiseman-Irvin column. Some of its advantages and limitations are discussed.

The altered procedure used in preparing columns is as follows: The solvents employed are acetone and hexane. The substitution of high-purity hexane for Skellysolve B reduces the blank titer values essentially to zero without solvent repurification. All solvents except 1% acetone are equilibrated for 2 to 3 min with a solution containing 25 ml of 50% sucrose solution, four drops of cresol red indicator, and 1 ml of saturated Ba(OH)₂ for each liter of solvent.

¹ Authorized for publication on April 22, 1963, as Paper No. 2768 in the Journal series of the Pennsylvania Agricultural Experiment Station.

² Present address: Department of Dairy Science, University of New Hampshire, Durham.