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Organic Acids in Fresh Bovine Semen

Department of Dairy Science, University of Illinois, Urbana

ABSTRACT

An organic acid analysis of fresh bovine semen has shown that acetic, lactic, glycolic, and citric acids are major constituents of this material. Propionic, formic, succinic, malonic, and malic acids were found in measurable quantity in a pooled semen sample, with only a trace of β-hydroxybutyric acid. With individual ejaculates, acetic and lactic acids were in the greatest quantity, followed by formic and propionic. β-hydroxybutyric acid was not detected and the other acids were not determined in the single ejaculates. The concentration of these acids, with the exception of β-hydroxybutyric, was greater in semen than in bovine blood. Presence of these acids in semen may influence results from aerobic metabolism studies, and the technique used for determining organic acids may be useful in studying oxidative pathways.