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Absorption from the Omasum and Subsequent Metabolism of Butyrate and Acetate¹

Department of Dairy Science, The Pennsylvania State University, University Park

ABSTRACT

Labeled sodium salts of butyric or acetic acids were injected into the exteriorized omasum of four calves, with the objective of further characterizing absorption from that organ. Changes in, and subsequent use of, the absorbed butyrate also were studied. Organic acid levels and distribution of C¹⁴ activity were determined in blood drawn simultaneously from the omasal vein and carotid artery at four times during 15 min following injection. The animals then were sacrificed and similar determinations made on stomachs' ingesta. Butyrate-C¹⁴ rapidly disappeared from the omasum. There was evidence of limited conversion of butyrate to other volatile fatty acids, both in the ingesta of the omasum and in the blood. The major portion of the C¹⁴ from butyrate appeared in the blood as betahydroxybutyrate (BHBA) in close association with blood lactate. BHBA was separated from lactate by first preparing hydroxamate derivatives, then employing chromatographic-autoradiographic procedures. Most of the C¹⁴ appeared in BHBA, regardless of whether the label had been on the first or third carbon of injected butyrate. Considerable radioactivity was found in blood bicarbonates following butyrate-1-C¹⁴ injection. Acetate was absorbed and appeared in the blood.

¹ Authorized for publication on April 22, 1963, as Paper No. 2767 in the Journal series of the Pennsylvania Agricultural Experiment Station.

² Present address: Department of Animal Husbandry, University of California, Davis.

³ Present address: Department of Dairy Science, University of New Hampshire, Durham.