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Department of Physiology, Veterinary College, Hannover, Germany
ABSTRACT
Usually, the contents are not equally distributed throughout the rumen (4, 9, 14). Particularly, stratification occurs after feeding. In cattle, this can be found up to 20 hr after feeding (2). As a consequence, an unequal distribution of single constituents—e.g., dry matter, VFA, microorganisms—persists (3, 15, 16).
This condition makes it difficult to obtain representative samples. The problem of obtaining good samples with an esophageal tube has been discussed by Hobson (11). Samples taken through a rumen fistula are more satisfactory (6), especially when contents taken from different parts of the rumen are mixed for the sample (13). In small ruminants, the formation of layers within the rumen seems to be less pronounced (5).
Incomplete and slow mixing interferes also with the distribution of indicator substances in the rumen. Therefore, it has not been possible to follow up the more rapidly occurring metabolic or regulatory processes with the aid of such indicators.
1 Supported by a grant of the Deutsche Forschungsgemeinschaft.
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